Obesity Is a Related Factor With Severe COVID-19 Pneumonia in Children: A Case Series

A small proportion of children with coronavirus disease 2019 (COVID-19) develop severe pneumonia. We reported the severe COVID-19 pneumonia in children aged <18 years with COVID-19 treated with remdesivir and dexamethasone from August 2021 to November 2021 by a retrospective review of their medical records. Eight children were included (5 males and 3 females). Their median age was 16 years (range: 9 to 17 years) and their median body mass index was 30.5 kg/m2 (range: 22.8–38.5 kg/m2). All patients had fever and dyspnea with hypoxia. Chest radiographic findings were variable and included diffuse or multifocal consolidation, nodules, and ground-glass opacities in the lungs. After receiving intravenous remdesivir and dexamethasone, 7 of the 8 children experienced an improvement in their symptoms within 2 days. In our case series, obesity was present in 6 out of 8 children with severe COVID-19 pneumonia.

High-affinity, neutralizing antibodies to SARS-CoV-2 can be made without T follicular helper cells

[Figure: see text].

Adaptive immune determinants of viral clearance and protection in mouse models of SARS-CoV-2

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused more than 160 million infections and more than 3 million deaths worldwide. Although effective vaccines are currently being deployed, the adaptive immune determinants that promote viral clearance and confer protection remain poorly defined. Using mouse models of SARS-CoV-2, we demonstrate that both humoral and cellular adaptive immunity contribute to viral clearance in the setting of primary infection. Furthermore, we find that either convalescent mice or mice that receive mRNA vaccination are protected from both homologous infection and infection with a variant of concern, B.1.351. In addition, we find that this protection is largely mediated by antibody response and not cellular immunity. These results highlight the in vivo protective capacity of antibodies generated to both vaccine and natural infection.

Effects of personal hygiene habits on self-efficacy for preventing infection, infection-preventing hygiene behaviors, and product-purchasing behaviors

Since there is no cure for the COVID-19 pandemic yet, personal hygiene management is important for protecting oneself from the deadly virus. Personal hygiene management comes from personal hygiene habits. Thus, this study investigated the association between personal hygiene habits, consumers’ infection-prevention behaviors, and the effects of social support on the latter. Data were collected using a self-administered questionnaire survey of 620 Korean adults. An online survey agency was used to conduct the questionnaire over eight days, from 18 May to 25 May, 2020. Data were analyzed using structural equation modeling. The results were as follows. First, personal hygiene habits positively affected self-efficacy for infection prevention (β = 0.123, p < 0.01). Moreover, personal hygiene habits indirectly affected virus spread-prevention behaviors (β = 0.457, p < 0.000) and product-purchasing behaviors for infection prevention (β = 0.146, p < 0.01) through self-efficacy for infection prevention. Second, informational support for infection prevention increased self-efficacy influence for infection prevention on the virus spread prevention behaviors among the public (composite reliability: −2.627). Thus, continued education of the public is imperative to ensuring compliance with personal hygiene practices. Furthermore, timely dissemination of relevant information on infection-prevention practices through various media during an infection outbreak is critical. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Divergent and self-reactive immune responses in the CNS of COVID-19 patients

Background: One third of COVID-19 patients develop significant neurological symptoms, yet SARS-CoV-2 is rarely detected in central nervous system (CNS) tissue, suggesting a potential role for parainfectious processes, including neuroimmune responses. Methods: We examined immune parameters in CSF and blood samples from a cohort of hospitalized patients with COVID-19 and significant neurological complications (n=6), compared to SARS-CoV-2 uninfected controls (Fig1A). Immune cells were characterized by single cell RNA and repertoire sequencing. Intrathecal antibodies were assessed for anti-viral and auto-reactivity by ELISA, mouse brain immunostaining, phage display, and IP-MS. Results: Through single cell and parallel cytokine analyses of CSF and paired plasma, we found divergent T cell responses in the CNS compartment, including increased levels of IL-1B and IL-12-associated innate and adaptive immune cell activation (Fig1B). We found evidence of clonal expansion of B cells in the CSF, with B cell receptor sequences that were unique from those observed in peripheral blood B cells (Fig1C), suggesting a divergent intrathecal humoral response to SARS-CoV-2. Indeed, all COVID-19 cases examined had anti-SARS-antibodies. Next, we directly examined whether CSF resident antibodies targeted self-antigens and found a significant burden of CNS autoimmunity, with the CSF from most patients recognizing neural self-antigens. COVID-19 CSF produced immunoreactive staining of specific anatomic regions of the brain including cortical neurons, olfactory bulb, thalamus, and cerebral vasculature. Finally, we produced a panel of monoclonal antibodies from patients' CSF and peripheral blood, and show that these target both anti-viral and anti-neural antigens-including one CSF-derived mAb specific for the spike protein that also recognizes neural tissue (Fig1D). Conclusion: This immune survey reveals evidence of a compartmentalized and self-reactive immune response in the CNS in COVID-19 patients with neurologic symptoms. We identified both innate and adaptive anti-viral immune responses, as well as humoral autoimmunity that appears to be unique to the CNS during SARS-CoV-2 infection. These data suggest a potential role for autoimmunity in contributing to neurological symptoms, and merit further investigation to the potential role of autoantibodies in post-acute COVID-19 neurological symptoms.

Design and development of learning mathematics game for primary school using handheld augmented reality

The traditional learning methods have some drawbacks and limitation such as restricted learning space, high cost, and lack of student engagement. In the era of digitalization, traditional education starts to transform into a more dynamic and modern education with the influence of the 21st-century learning method. Moreover, due to the pandemic, schools and universities are moving to digital learning to contain the spread of Coronavirus Disease 2019 (COVID-19). Therefore, the aim of this project is to develop a Mathematics game using handheld Augmented Reality (HAR) for primary school children as an alternative method in learning Mathematics. There are three main phases have been carried out, first is to study the requirements of an educational game. The second phase involves the design and development including the game element, storyline, game characters as well as user interface design. Finally, the third phase is integrating the learning Mathematics game using HAR, followed by evaluation. The evaluation of the learning Mathematics game was carried out for usability and user acceptance. The usability results show that the users are satisfied with the prototype and feedbacks show the game is fun. Overall, this project has successfully produced a learning Mathematics game using HAR for primary school children. © Published under licence by IOP Publishing Ltd.